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[Bioinformatics]
[Biochemistry]
[Genes and Development]
[Genetics]
[Molecular Biology]
[Recombinant DNA Techniques]
[Virology]
[Protocols]
[Research]

General protocols

bulletLab writeups [4/30/03]

Cloning

bulletCloning blunt ended PCR fragments (ZeroBlunt) [5/7/09]
bulletCloning standard PCR fragments (TopoTA) [9/30/04]

DNA analysis

bulletAgarose gels-TBE [3/7/07]
bulletAgarose gels-TAE [3/7/07]
bulletDNA ladders [2/16/06]
bulletPCR (FideliTaq or AmpliTaq) [9/14/05]

DNA purification

bulletClontech minipreps [11/24/08]
bulletQiagen minipreps [3/19/04]
bulletQiagen minipreps-Thermoplasma [4/30/03]
bulletSoil DNA purification [4/30/03]

DNA sequencing

bulletABI 377 procedures
 
bulletPCR sequencing [9/30/04]
bullet Sequencing cleanup-Centrisep [10/23/03]
bullet Sequencing-place on combs [7/9/03]
bulletSequencing (all three procedures) [9/30/04]
 
bulletBeckman procedures
 
bullet Templiphi amplification of plasmids [9/12/06]
bullet PCR sequencing-Templiphi templates [9/12/06]
bullet PCR sequencing-Miniprep templates [1/8/10]
bullet Ethanol precipitation and sequencing [1/8/10]

 

bulletGeneral procedures
 
bulletBLAST searches for homologies [7/9/03]

Mammalian cell culture

bulletFeLV cell cultures [9/19/03]
bulletFeLV 6-well plates [11/7/03]

RNA

bulletRT-PCR Invitrogen [3/22/07]
bullet RT-PCR Takara BluePrint [1/22/10]

 Last updated on: January 22, 2010

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Copyright 2010 by Dr. Dennis Revie, all rights reserved.
For problems or questions regarding this web contact: revie@clunet.edu.